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Registro Completo |
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
28/09/1995 |
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Data da última atualização: |
07/04/2017 |
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Autoria: |
WEST, A. W.; SPARLING, G. P. |
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Título: |
Modifications to the substrate-induced respiration method to permit measurement of microbial biomass in soils of differing water contents. |
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Ano de publicação: |
1986 |
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Fonte/Imprenta: |
Journal Microbiological Methods, v.5, p.177-189, 1986. |
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Idioma: |
Inglês |
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Conteúdo: |
An improvement to the substrate-induced respiration (SIR) method of Anderson and Domsch is described. In the original procedure endogenous CO2 respiration of soil micro-organisms was stimulated by addition of glucose which removed the substrate limitation to soil microbial respiration. However, the water limitation to soil microbial respiration was not removed. The modification proposed here are (1) to dissolve glucose in water prior to addition to soil and (2) to add glucose to soil on a concentration (weight of glucose/volume of water) basis to achieve a ratio of 2 ml glucose solution to 1 gram dry weight of soil. Thus, both substrate and water limitations to respiration are allevfiated. Data are presented which asses these modifications including shape and size of the vessel used to contain soil, optimal CO2 equilibration between liquid and gaseous phases, effect of substrate concentration, sampling frequency and interference from viable root fragments. The improved method permits estimation of induced microbial respiration (using gas chromatography) in a rapid and simple manner on soils of any water content. Calibration with biovolume-derived biomass C estimates on three soils of varying water contents produced a linear relationships between log10 respiration rate and the biomass C. Biomass C determined by microscopy using non-vital stains probably included non-viable organisms which did not respire. From the above relationship, this non-respiring portion of the (biovolume-derived) biomass was calculated to be 59 ug C g-1 soil. MenosAn improvement to the substrate-induced respiration (SIR) method of Anderson and Domsch is described. In the original procedure endogenous CO2 respiration of soil micro-organisms was stimulated by addition of glucose which removed the substrate limitation to soil microbial respiration. However, the water limitation to soil microbial respiration was not removed. The modification proposed here are (1) to dissolve glucose in water prior to addition to soil and (2) to add glucose to soil on a concentration (weight of glucose/volume of water) basis to achieve a ratio of 2 ml glucose solution to 1 gram dry weight of soil. Thus, both substrate and water limitations to respiration are allevfiated. Data are presented which asses these modifications including shape and size of the vessel used to contain soil, optimal CO2 equilibration between liquid and gaseous phases, effect of substrate concentration, sampling frequency and interference from viable root fragments. The improved method permits estimation of induced microbial respiration (using gas chromatography) in a rapid and simple manner on soils of any water content. Calibration with biovolume-derived biomass C estimates on three soils of varying water contents produced a linear relationships between log10 respiration rate and the biomass C. Biomass C determined by microscopy using non-vital stains probably included non-viable organisms which did not respire. From the above relationship, this non-respiring portion of the (biovolu... Mostrar Tudo |
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Thesagro: |
Biomassa; Solo. |
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Thesaurus Nal: |
biomass; soil. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02094naa a2200181 a 4500
001 1786371
005 2017-04-07
008 1986 bl --- 0-- u #d
100 1 $aWEST, A. W.
245 $aModifications to the substrate-induced respiration method to permit measurement of microbial biomass in soils of differing water contents.
260 $c1986
520 $aAn improvement to the substrate-induced respiration (SIR) method of Anderson and Domsch is described. In the original procedure endogenous CO2 respiration of soil micro-organisms was stimulated by addition of glucose which removed the substrate limitation to soil microbial respiration. However, the water limitation to soil microbial respiration was not removed. The modification proposed here are (1) to dissolve glucose in water prior to addition to soil and (2) to add glucose to soil on a concentration (weight of glucose/volume of water) basis to achieve a ratio of 2 ml glucose solution to 1 gram dry weight of soil. Thus, both substrate and water limitations to respiration are allevfiated. Data are presented which asses these modifications including shape and size of the vessel used to contain soil, optimal CO2 equilibration between liquid and gaseous phases, effect of substrate concentration, sampling frequency and interference from viable root fragments. The improved method permits estimation of induced microbial respiration (using gas chromatography) in a rapid and simple manner on soils of any water content. Calibration with biovolume-derived biomass C estimates on three soils of varying water contents produced a linear relationships between log10 respiration rate and the biomass C. Biomass C determined by microscopy using non-vital stains probably included non-viable organisms which did not respire. From the above relationship, this non-respiring portion of the (biovolume-derived) biomass was calculated to be 59 ug C g-1 soil.
650 $abiomass
650 $asoil
650 $aBiomassa
650 $aSolo
700 1 $aSPARLING, G. P.
773 $tJournal Microbiological Methods$gv.5, p.177-189, 1986.
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Registro original: |
Embrapa Pantanal (CPAP) |
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